Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 847-852, 2014.
Article
en En
| WPRIM
| ID: wpr-812192
Biblioteca responsable:
WPRO
ABSTRACT
AIM@#A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2.@*METHODS@#Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2. The monoclonal antibody was purified by protein G affinity, whereas the polyclonal antibody was purified by protein A affinity. The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples.@*RESULTS@#A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2, highly specific monoclonal and polyclonal antibodies. This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples.@*CONCLUSIONS@#The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit.
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Texto completo:
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Índice:
WPRIM
Asunto principal:
Sangre
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Proteínas Recombinantes
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Ensayo de Inmunoadsorción Enzimática
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Receptores Inmunológicos
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Alergia e Inmunología
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Métodos
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Ratones Endogámicos BALB C
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Anticuerpos Monoclonales
Límite:
Animals
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Humans
Idioma:
En
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2014
Tipo del documento:
Article