Tetramethylpyrazine protects lymphocytes from radiation-induced apoptosis through nuclear factor-κB / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 730-737, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-812207
ABSTRACT
AIM@#Radiation induces an important apoptosis response in irradiated organs. The objective of this study was to investigate the radioprotective effect of tetramethylpyrazine (TMP) on irradiated lymphocytes and discover the possible mechanism of protection.@*METHOD@#Lymphocytes were pretreated for 12 h with TMP (25-200 μmol·L(-1)) and then exposed to 4 Gy radiation. Cell apoptosis and the signaling pathway were analyzed.@*RESULTS@#Irradiation increased cell death, DNA fragmentation, activated caspase activation and cytochrome c translocation, downregulated B-cell lymphoma 2 (Bcl-2) and up-regulated Bcl-2-associated X protein (Bax). Pretreated with TMP significantly reversed this tendency. Several anti-apoptotic characteristics of TMP, including the ability to increase cell viability, inhibit caspase-9 activation, and upregulate Bcl-2 and down-regulate Bax in 4Gy-irradiated lymphocytes were determined. Signal pathway analysis showed TMP could translate nuclear factor-κB (NF-κB) from cytosol into the nucleus.@*CONCLUSION@#The results suggest that TMP had a radioprotective effect through the NF-κB pathway to inhibit apoptosis, and it may be an effective candidate for treating radiation diseases associated with cell apoptosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Pirazinas
/
Efectos de la Radiación
/
Protectores contra Radiación
/
Medicamentos Herbarios Chinos
/
Linfocitos
/
Línea Celular
/
Supervivencia Celular
/
FN-kappa B
/
Apoptosis
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2014
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS