Crebanine inhibits voltage-dependent Na+ current in guinea-pig ventricular myocytes / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 20-23, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-812314
ABSTRACT
AIM@#To study the effects of crebanine on voltage-gated Na(+) channels in cardiac tissues.@*METHODS@#Single ventricular myocytes were enzymatically dissociated from adult guinea-pig heart. Voltage-dependent Na(+) current was recorded using the whole cell voltage-clamp technique.@*RESULTS@#Crebanine reversibly inhibited Na(+) current with an IC50 value of 0.283 mmol·L(-1) (95% confidence range 0.248-0.318 mmol·L(-1)). Crebanine at 0.262 mmol·L(-1) caused a negative shift (about 12 mV) in the voltage-dependence of steady-state inactivation of Na(+) current, and retarded its recovery from inactivation, but did not affect its activation curve.@*CONCLUSION@#In addition to blocking other voltage-gated ion channels, crebanine blocked Na(+) channels in guinea-pig ventricular myocytes. Crebanine acted as an inactivation stabilizer of Na(+) channels in cardiac tissues.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Aporfinas
/
Farmacología
/
Medicamentos Herbarios Chinos
/
Regulación hacia Abajo
/
Células Cultivadas
/
Química
/
Biología Celular
/
Miocitos Cardíacos
/
Stephania
/
Canales de Sodio Activados por Voltaje
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2014
Tipo del documento:
Artículo
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