Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 175-183, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-812415
ABSTRACT
Given the limited studies and conflicting findings, the transport character of ginsenosides crossing the blood-brain barrier (BBB) remains unclear. The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry. In brain tissue homogenates, ginsenoside Rg1 was detectable and no other ginsenosides or their metabolites were found. No ginsenosides were detected in cerebrospinal fluid. Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues. Furthermore, immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes, and in few neurons. These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB, although the transport capability of ginsenosides through the BBB may be poor.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Encéfalo
/
Química Encefálica
/
Medicamentos Herbarios Chinos
/
Barrera Hematoencefálica
/
Química
/
Administración Oral
/
Cromatografía Líquida de Alta Presión
/
Ratas Sprague-Dawley
/
Ginsenósidos
/
Espectrometría de Masas en Tándem
Tipo de estudio:
Estudio diagnóstico
/
Investigación cualitativa
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Journal of Natural Medicines (English Ed.)
Año:
2018
Tipo del documento:
Artículo
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