Protective effects of rosiglitazone on hepatic ischemia reperfusion injury in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 732-737, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-813203
ABSTRACT
To explore the protective effect of rosiglitazone (RGZ) on hepatic ischemia reperfusion injury (HIRI) and the underlying mechanisms.
Methods:
A rat model of ischemia-reperfusion injury was established by clamping the left and middle lobe of liver with noninvasive vascular clamp. A total of 30 Sprague-Dawley rats were randomly divided into a sham group, an HIRI group, and a RGZ group (10 rats in each group). Two hours after reperfusion, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, lactate dehydrogenase (LDH) level, malondialdehyde (MDA) content and catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were examined. HE staining was used to observe liver pathological morphology. The liver peroxisome proliferators-activated receptor γ (PPAR-γ), p-PPAR-γ, nuclear factor related factor 2 (Nrf-2), antioxidant response element (ARE), heme oxygenase 1 (HO-1) and quinone oxidoreductase-1 (NQO-1) were detected by Western blot.Results:
Compared with the HIRI group, the levels of ALT, AST, LDH and MDA in the RGZ group were significantly decreased (all P0.05).Conclusion:
PPAR-γ agonist RGZ can attenuate HIRI, which may be related to activating Nrf2/ARE signaling pathway and enhancement of antioxidant ability.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Aspartato Aminotransferasas
/
Superóxido Dismutasa
/
Sangre
/
Daño por Reperfusión
/
Catalasa
/
Distribución Aleatoria
/
Ratas Sprague-Dawley
/
Tiazolidinedionas
/
Usos Terapéuticos
/
Alanina Transaminasa
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2018
Tipo del documento:
Artículo
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