Effects of vasoactive intestinal peptide on LPS-induced MMP-9 expression by alveolar macrophages in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 645-649, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-813457
ABSTRACT
OBJECTIVE@#To explore the role of vasoactive intestinal peptide (VIP) on LPS-induced MMP-9 expression by alveolar macrophages (AM) in rats.@*METHODS@#LPS-induced cultured Wistar rats AMs were treated with different concentrations of VIP (10(-10) to approximately 10(-6) mol/L) for 24 h. AMs and the supernatant were collected to measure the MMP-9 expression and activity by RT-PCR and gelatin zymography, respectively. Results The MMP-9 activity and expression of LPS-induced AMs were significantly higher than those in the control group (P < 0.01). VIP (10(-9) to approximately 10(-6) mol/L) down-regulated LPS-induced MMP-9 activity and its expression. The effects were diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (P < 0.01).@*CONCLUSION@#VIP can decrease LPS-induced MMP-9 activity and its expression, which may be related to protein kinase C and calmodulin pathway. VIP may have protective roles in the lung injury.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Proteína Quinasa C
/
Calmodulina
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Péptido Intestinal Vasoactivo
/
Regulación hacia Abajo
/
Células Cultivadas
/
Lipopolisacáridos
/
Macrófagos Alveolares
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Ratas Wistar
/
Metaloproteinasa 9 de la Matriz
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2005
Tipo del documento:
Artículo
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