Construction and bio-activity of the chimeric protein of BMP2-EGFP / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 16-20, 2005.
Article
en Zh
| WPRIM
| ID: wpr-813484
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To construct the recombined retroviral expression vector of BMP2/pLEGFP and investigate the bio-activity of the expressed chimeric protein.@*METHODS@#The recombinant vector constructed by gene recombinant technology was analyzed by restriction enzyme digestion and PCR. BMP2/pLEGFP was transfected into COS-7 cells with liposome transfection reagents for transient expression. The expression of chimeric protein BMP2/EGFP was identified by fluorescent microscope and Western blotting. The bio-activity was examined by the cellular activity and animal heterotopic osteogenesis experiment.@*RESULTS@#The recombinant plasmid proved successful by restriction enzyme digestion and PCR. The expression of the chimeric protein was shown by fluorescent microscope and Western blotting. The chimeric protein had the double bio-activities of BMP2 and EGFP identified by the cellular activity and animal heterotopic osteogenesis tests.@*CONCLUSION@#The recombinant vector of BMP2/pLEGFP is successfully constructed by the gene recombinant technology and its chimeric protein has double bioactivities of BMP2 and EGFP.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
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Transfección
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Chlorocebus aethiops
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Factor de Crecimiento Transformador beta
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Células COS
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Proteínas Morfogenéticas Óseas
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Proteínas Fluorescentes Verdes
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Células Eucariotas
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Proteína Morfogenética Ósea 2
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Genética
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2005
Tipo del documento:
Article