Rapid determination of optimal time for peripheral blood stem cell harvest by detecting hematopoietor progenitor cell during mobilization / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 570-573, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-813503
ABSTRACT
OBJECTIVE@#To develop a simple method to rapidly determine the optimal time of perpheral blood stem cell harvest.@*METHODS@#We selected 5 healthy donors mobilized with granulocyte colony-stimulating factor (G-CSF) in allogenic peripheral blood stem cell transplantation (allo-PBSCT) to continuously monitor the variety of hematopoietic stem/progenitor cells (HSC/HPC) in the peripheral blood. HPC in the peripheral blood was detected by Sysmex XE-2100 fully automated hematology analyzer with an immature myeloid information channel and the CD34+ cells and colony-forming unit-granulocyte-macrophage (CFU-GM) were determined by flow cytometry and colony culture during mobilization.@*RESULTS@#HPC was positively correlated with the CD34+ cells or CFU-GM in the peripheral blood (r = 0.82 or r = 0.85, respectively). HPC, CD34+ cells, and CFU-GM in allo-PBSCT donors obviously increased on the 4th day after the mobilization, and their peaks were on the 5th day after the mobilization. But HPC varied more than CD34+ cells and CFU-GM did.@*CONCLUSION@#Sysmex XE-2100 can rapidly monitor the variety of HSC/HPC in allo-PBSCT donors mobilizated with G-CSF, and can provide a simple and economic method to determine the optimal time of peripheral blood stem cell harvest.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Tiempo
/
Proteínas Recombinantes
/
Células Madre Hematopoyéticas
/
Recuento de Células
/
Factor Estimulante de Colonias de Granulocitos
/
Trasplante de Células Madre Hematopoyéticas
/
Movilización de Célula Madre Hematopoyética
/
Biología Celular
/
Usos Terapéuticos
/
Métodos
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS