Cloning and Expression of the Gene Encoding the 32-kDa Protein of Rickettsia typhi
Journal of the Korean Society for Microbiology
; : 399-404, 1997.
Article
en Ko
| WPRIM
| ID: wpr-81357
Biblioteca responsable:
WPRO
ABSTRACT
The crystalline surface layer protein (SLP) and a 28-32 kDa antigen of Rickettsia typhi were known as strong immunogens. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. Our study also showed that a 48-kDa protein reacted strongly with polyclonal antiserum of a patient with murine typhus. In this study, we produced three recombinant proteins (SLP, 32-kDa, and 48-kDa protein) in E. coli as fusion proteins with maltose binding proteins. The reactivity of these proteins with patients' sera was investigated.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Rickettsia
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Rickettsia typhi
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Proteínas Recombinantes
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Tifus Endémico Transmitido por Pulgas
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Sistemas de Lectura Abierta
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Células Clonales
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Análisis de Secuencia
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Clonación de Organismos
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Cristalinas
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Proteínas de Unión a Maltosa
Límite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Society for Microbiology
Año:
1997
Tipo del documento:
Article