Effects of advanced glycation end products on the activity of NF-kappaB and the expression of fibronectin mRNA in the endothelial cells in aged rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 883-887, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-813581
ABSTRACT
OBJECTIVE@#To explore the effects of advanced glycation end products (AGEs) on the activity of NF-kappaB and fibronectin (Fn) synthesis in the endothelial cells in aged rats.@*METHODS@#Endothelial cells were cultured in M199 from the aorta of 24 month old rats and divided into 3 groups Group A (5 mmol/L glucose) as controls, Group B (25 mg/L AGEs for 48 h), and Group C (50 mg/L AGEs for 48 h). The activity of NF-kappaB was evaluated by immunofluorescence and the expression of Fn mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR).@*RESULTS@#Compared with the controls, AGEs induced the activity of NF-kappaB and increased the Fn mRNA expression in a concentration-dependent manner (P<0.05).@*CONCLUSION@#The activity of NF-kappaB and up-regulated expression of Fn mRNA induced by AGEs may contribute to chronic complications of diabetes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
ARN Mensajero
/
Células Cultivadas
/
FN-kappa B
/
Fibronectinas
/
Ratas Sprague-Dawley
/
Productos Finales de Glicación Avanzada
/
Células Endoteliales
/
Genética
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2006
Tipo del documento:
Artículo
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