Sorcin overexpression and multidrug resistance in human gastric cancer cells / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To screen multidrug resistance (MDR) related proteins in human gastric cancer using proteomics technique.@*METHODS@#Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant human gastric cancer cell line SGC7901/VCR and its counterpart SGC7901. PDQuest software was used to analyze 2-DE images, and the differential expression proteins between the 2 cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF. The differential expression level of sorcin, one of the identified proteins, was confirmed by western blot analysis. The effect of sorcin on the development of MDR of SGC7901/VCR was determined by antisense oligonucleotides (ASO) technique.@*RESULTS@#Sorcin as a high expression protein in SGC7901/VCR was identified and the suppression of sorcin expression by sorcin ASO could enhance the vincristine chemosensitivity in SGC7901/VCR.@*CONCLUSION@#Sorcin overexpression is related to MDR in human gastric cancer cell line SGC7901/VCR.