Sorcin overexpression and multidrug resistance in human gastric cancer cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 340-349, 2006.
Article
en Zh
| WPRIM
| ID: wpr-813702
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To screen multidrug resistance (MDR) related proteins in human gastric cancer using proteomics technique.@*METHODS@#Two-dimensional electrophoresis (2-DE) was used to separate the total proteins of vincristine-resistant human gastric cancer cell line SGC7901/VCR and its counterpart SGC7901. PDQuest software was used to analyze 2-DE images, and the differential expression proteins between the 2 cell lines were identified by both MALDI-TOF-MS and ESI-Q-TOF. The differential expression level of sorcin, one of the identified proteins, was confirmed by western blot analysis. The effect of sorcin on the development of MDR of SGC7901/VCR was determined by antisense oligonucleotides (ASO) technique.@*RESULTS@#Sorcin as a high expression protein in SGC7901/VCR was identified and the suppression of sorcin expression by sorcin ASO could enhance the vincristine chemosensitivity in SGC7901/VCR.@*CONCLUSION@#Sorcin overexpression is related to MDR in human gastric cancer cell line SGC7901/VCR.
Texto completo:
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Índice:
WPRIM
Asunto principal:
Farmacología
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Neoplasias Gástricas
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Vincristina
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Proteínas de Unión al Calcio
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Datos de Secuencia Molecular
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Electroforesis en Gel Bidimensional
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Secuencia de Aminoácidos
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Resistencia a Múltiples Medicamentos
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Resistencia a Antineoplásicos
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Línea Celular Tumoral
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2006
Tipo del documento:
Article