Protective effect of thrombin precondition on astrocytes insulted by oxygen-glucose deprivation / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 845-849, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-813788
ABSTRACT
OBJECTIVE@#To explore the effect of thrombin precondition (TPC) on the rat cerebral astrocytes(As) cultured in oxygen-glucose deprivation (OGD).@*METHODS@#Astrocytes were pretreated with thrombin (TB) at various concentrations (0.005 approximately 5.000 kU/L), and then insulted by OGD. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) effusion rate and the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry technique. The glutamate uptake of astrocytes was studied with [3H]-glutamate incorporation.@*RESULTS@#OGD increased the LDH, decreased the cell viability, increased the number of apoptotic astrocytes, and decreased the glutamate uptake (P<0.01). While preconditioned with thrombin at the same condition, the LDH decreased, the cell viability increased, the percentage of apoptotic cells decreased, and the glutamate uptake increased (P<0.05). The maximum protective effect of thrombin was observed at 0.1 kU/L.@*CONCLUSION@#Low concentration of thrombin precondition (TPC) can protect the astrocytes from oxygen-glucose deprived injury, and attenuate its apoptosis in a dose-dependent manner.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxígeno
/
Farmacología
/
Trombina
/
Hipoxia de la Célula
/
Células Cultivadas
/
Astrocitos
/
Ratas Sprague-Dawley
/
Apoptosis
/
Fármacos Neuroprotectores
/
Ácido Glutámico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2007
Tipo del documento:
Artículo
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