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Effect of rosiglitazone on NO and eNOS via PI3K/PKB signal pathways in cultured human umbilical vein endothelial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 824-830, 2007.
Artículo en Chino | WPRIM | ID: wpr-813792
ABSTRACT
OBJECTIVE@#To observe the effect of rosiglitazone on the production of nitric oxide (NO) and the expression of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) /the endothelial nitric oxide synthase (eNOS) in cultured human umbilical vein endothelial cells(HUVECs), and to investigate the mechanism of signal transduction of rosiglitazone in improving the endothelial function.@*METHODS@#HUVECs were treated with various concentrations of rosiglitazone. The NO level was measured using Griess Reaction in cell culture supernatants; the expressions of PI3K-, PKB- and eNOS mRNA were measured using RT-PCR; and the expressions of PKB, eNOS, and phosphorylation of PKB-Ser473, eNOS-Ser1177 were measured using Western Blot.@*RESULTS@#Rosiglitazone increased the endothelial NO production in a dose- and time-dependent manner in cultured HUVECs, and also increased the expression of PI3K mRNA and the phosphorylation of PKB-Ser473 and eNOS-Ser1177 in a concentration-dependent manner, with no alteration in the expression of PKB and eNOS in cultured HUVECs. N(w)-nitro-L- arginine methyl ester (L-NAME, eNOS synthase inhibitor) blocked the rosiglitazone-induced NO formation; LY294002 (a PI3K inhibitor) prevented the NO production; and the phosphorylation of eNOS and PKB was induced by rosiglitazone.@*CONCLUSION@#Treatment with rosiglitazone can increase the NO production and improve the endothelial function through up-regulating the PI3K/PKB/eNOS signal pathways in cultured HUVECs.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fosforilación / Transducción de Señal / Células Cultivadas / Fosfatidilinositol 3-Quinasas / Tiazolidinedionas / Óxido Nítrico Sintasa de Tipo III / Proteínas Proto-Oncogénicas c-akt / Células Endoteliales de la Vena Umbilical Humana / Rosiglitazona Límite: Humanos Idioma: Chino Revista: Journal of Central South University(Medical Sciences) Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fosforilación / Transducción de Señal / Células Cultivadas / Fosfatidilinositol 3-Quinasas / Tiazolidinedionas / Óxido Nítrico Sintasa de Tipo III / Proteínas Proto-Oncogénicas c-akt / Células Endoteliales de la Vena Umbilical Humana / Rosiglitazona Límite: Humanos Idioma: Chino Revista: Journal of Central South University(Medical Sciences) Año: 2007 Tipo del documento: Artículo