Effects of endothelial cells from cord blood on the amplification of human early hematopoietic cells from cord blood in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 304-308, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-813889
ABSTRACT
OBJECTIVE@#To observe the effects of endothelial cells from umbilical cord blood (UCB) on the amplification of human early hematopoietic cells from UCB in vitro.@*METHODS@#Endothelial cells from UCB were cultured by the optimized medium of endothelial cells. There were 2 experiment groups cytokines group (SCF+IL-3+IL-6+GM-CSF, CKs group) and noncontact group (endothelial cell layer with CKs without contacting the CD34+ cells group). CD34+ cells from UCB were isolated by MiniMACS. After the cells in the CKs group and the noncontact group were cultured for 7 days, the amplifying folds of early hematopoietic cells were assayed.@*RESULTS@#Early hematopoietic cells from UCB were expanded in the CKs group or the noncontact group. The amplifying folds of the noncontact group on early hematopoietic cells were significantly more than those of the CKs group.@*CONCLUSION@#The amplification effect of the noncontact group on early hematopoietic cells is superior to that of the CKs group.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Células Madre Hematopoyéticas
/
Inmunohistoquímica
/
Citocinas
/
Técnicas de Cocultivo
/
Antígenos CD34
/
Medios de Cultivo
/
Biología Celular
/
Células Endoteliales
/
Proliferación Celular
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2007
Tipo del documento:
Artículo
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