PTX1 in nasopharyngeal carcinoma by RNAi technology / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 235-240, 2007.
Article
en Zh
| WPRIM
| ID: wpr-813901
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the expression and the role of PTX1 located at the amplified 12p12-p11 region in nasopharyngeal carcinoma (NPC).@*METHODS@#Semi-quantitative RT-PCR and real-time RT-PCR were applied to detect the expression level of PTX1 in 36 NPC and 8 chronic nasopharyngitis (NP) biopsies. RNAi vector targeting PTX1 was constructed and transfected into NPC cell line 6-10B. The RNAi effect was determined by detecting the expression level of PTX1 in transfected 6-10B cell line. Finally, the cell biological characteristics were compared between transfected 6-10B and parental 6-10B by analyzing the cell cycle distribution and apoptosis status using flow cytometry.@*RESULTS@#RT-PCR and real-time RT-PCR revealed that PTX1 gene was over-expressed in NPC tissues (P<0.05). PTX1 expression was suppressed in NPC cell line 6-10B by approximately 65% by RNAi, confirmed by RT-PCR. The depletion of PTX1 could effectively block the proliferation and induce the apoptosis of NPC cells.@*CONCLUSION@#Blocking the expression of PTX1 on mRNA level changed the characterization of NPC cell line 6-10B by RNAi, suggesting that PTX1 identified in the amplified 12p12-p11 region may be involved in the genesis and development of NPC via promoting the cell proliferation and inhibiting the cell apoptosis.
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Índice:
WPRIM
Asunto principal:
Patología
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Fisiología
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Carcinoma de Células Escamosas
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Transfección
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Regulación Neoplásica de la Expresión Génica
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Ciclo Celular
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Neoplasias Nasofaríngeas
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Apoptosis
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Proteínas de Transporte Vesicular
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2007
Tipo del documento:
Article