Function and gene expression of the active efflux transporters in drug-resistant Candida albicans / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 183-187, 2007.
Article
en Zh
| WPRIM
| ID: wpr-813910
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the function and gene expression of active efflux transporters in drug-resistant candida albicans.@*METHODS@#The broth microdilution method was performed to determined the minimal inhibitory concentration of 4 antifungal drugs to 20 candida albicans. We compared the efflux of Rhodamine 6G between sensitive and some of fluconazole-resistant candida albicans, and screened out the resistant strains with significantly increased efflux of Rhodamine 6G. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure the mRNA levels of active efflux gene CDR1 and CDR2.@*RESULTS@#The efflux of Rhodamine 6G was significantly increased in some fluconazole-resistant strains when glucose was added, and the gene expressions of CDR1 and CDR2 were also obviously increased, compared with those in the sensitive strains.@*CONCLUSION@#The excessive expression of active efflux pump gene is related to the resistance to fluconazole in candida albicans. The measurement of Rhodamine 6G efflux is a useful method for the identification of drug-resistant strains induced by the excessive expression of active efflux pump.
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Índice:
WPRIM
Asunto principal:
Proteínas de Transporte de Membrana
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Farmacología
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Fisiología
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Rodaminas
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Transporte Biológico
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Candida albicans
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ARN Mensajero
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Proteínas Fúngicas
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Pruebas de Sensibilidad Microbiana
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Fluconazol
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2007
Tipo del documento:
Article