Hyperlipidemia induced by high fat diet ingestion activates TGF-beta/Smad signaling pathway in the kidney of diabetic rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 906-912, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-813979
ABSTRACT
OBJECTIVE@#To investigate the effect of diet-induced hyperlipidemia on TGF-beta/Smad signaling pathway in the kidney of diabetic rats, and to explore the mechanism by which hyperlipidemia leads to renal injury in diabetes.@*METHODS@#Diabetic rats and non-diabetic rats were fed with normal fat diet and high fat diet for 16 weeks, respectively. The expressions of TGF-beta1, TbetaRII, and Col-IV mRNA in the renal cortex were examined by reverse transcriptase-PCR,TbetaRII and p-Smad staining in glomerular cells were detected by immunohistochemical staining, and the expression of TGF-beta1 and Col-IV protein was determined by Western blot.@*RESULTS@#Diet-induced hyperlipidemia up-regulated the levels of TGF-beta1, TbetaRII, p-Smad, and Col-IV protein and mRNA in the renal cortex of diabetic rats compared with those of non-diabetic rats. However, feeding high fat diet to non-diabetic rats had no influence on the expression of TGF-beta1, TbetaRII, p-Smad2, and Col-IV in the renal cortex.@*CONCLUSION@#Hyperlipidemia induced by high fat diet ingestion leads to renal injury in diabetic rats through activating TGF-beta1 /Smad signaling pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Grasas de la Dieta
/
Transducción de Señal
/
Ratas Sprague-Dawley
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Diabetes Mellitus Experimental
/
Nefropatías Diabéticas
/
Proteínas Smad
/
Factor de Crecimiento Transformador beta1
/
Hiperlipidemias
/
Riñón
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2008
Tipo del documento:
Artículo
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