Effect of FGF-2 on survivin and subcellular location of Smac in human small cell lung cancer cell NCI-H446 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 705-711, 2008.
Article
en Zh
| WPRIM
| ID: wpr-814011
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of basic fibroblast growth factor (FGF-2)on survivin and subcellular location of Smac in human small cell lung cancer (SCLC) cell NCI-H446.@*METHODS@#Western blot was used to detect the expression of survivin protein induced by FGF-2. The release of Smac from mitochondria to cytoplasm affected by FGF-2 was observed by Western blot and immunofluorescence. Apoptosis of NCI-H446 cells was detected with flow cytometry and Hoechst 33258 staining.@*RESULTS@#The expression of survivin could be up-regulated in response to FGF-2 treatment in NCI-H446 cells, and the level of survivin expression is related to the concentration and time of FGF-2 treatment. FGF-2 could inhibit the release of Smac from the mitochondria to cytoplasm induced by serum starving. FGF-2 could inhibit the apoptosis induced by serum starving.@*CONCLUSION@#FGF-2 up-regulates the expression of survivin protein in NCI-H446 cells, and blocks the release of Smac from mitochondria cytoplasm. Survivin and Smac might play important roles in the apoptosis inhibited by FGF-2.
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Asunto principal:
Patología
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Farmacología
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Células Tumorales Cultivadas
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Factor 2 de Crecimiento de Fibroblastos
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Apoptosis
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Citoplasma
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Proteínas Mitocondriales
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Péptidos y Proteínas de Señalización Intracelular
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Proteínas Reguladoras de la Apoptosis
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Proteínas Inhibidoras de la Apoptosis
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2008
Tipo del documento:
Article