Screening for differentially expressed proteins in nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 375-383, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-814068
ABSTRACT
OBJECTIVE@#To search for the differentially expressed proteins of nasopharyngeal carcinoma (NPC),and provide scientific evidence for identifying molecular biomarkers for NPC.@*METHODS@#Laser capture microdissection (LCM) was used to purify the target cells from NPC and normal nasopharyngeal epithelial tissues (NNET). Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins of microdissected NPC and NNET, PDQuest software was applied to analyze 2-DE images,and the differential proteins between the 2 types of tissues were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Western blot and immunohistochemistry of tissue microarray were used to detect the expression of the differential protein SCCA1 in NPC and NNET.@*RESULTS@#2-DE patterns of microdissected NPC and NNEC were established,and 36 differential proteins in the NPC and NNEC were identified,20 of which only expressed or up-regulated in NPC and 16 only expressed or up-regulated in NNET. The differentially expressed level of SCCA1 in the NPC and NNET was confirmed by Western blot and immunohistochemistry of tissue microarray.@*CONCLUSION@#Thirty-six differentially expressed proteins identified in this study may be associated with the carcinogenesis of NPC,and may be candidate molecular biomarkers for NPC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Datos de Secuencia Molecular
/
Carcinoma de Células Escamosas
/
Electroforesis en Gel Bidimensional
/
Biomarcadores de Tumor
/
Serpinas
/
Química
/
Neoplasias Nasofaríngeas
/
Secuencia de Aminoácidos
/
Proteómica
/
Microdisección
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2008
Tipo del documento:
Artículo
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