Effect of suppressive oligodeoxynucleotides on the functional differentiation in CD4(+) Th1 lymphocytes in mice in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1089-1094, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-814145
ABSTRACT
OBJECTIVE@#To explore the effect of suppressive oligodeoxynucleotides (Sup ODN) on the Th1 differentiation of CD4(+)T splenetic lymphocytes in mice.@*METHODS@#The splenetic lymphocytes of BALB/c mice were separated, and then CD4(+) cells were purified with immune magnetic CD4(+) microbeads (positive selection). The purification was examined by fluorescence-activated cell sorter. CD4(+) cells, anti-CD3epsilon, anti-CD28, IL-12 and Sup ODN or control oligodeoxynucleotides (Con ODN) were co-incubated for 72 h. IFN-gamma and IL-4 in the supernatant were detected using enzyme-linked immunosorbent assay. The expression of T-bet mRNA in CD4(+) cells was tested by reverse transcription polymerase chain reaction.@*RESULTS@#Sup ODN could significantly inhibit the release of INF-gamma and increase IL-4 production respectively (P<0.01). T-bet mRNA of CD4(+) lymphocytes was remarkably inhibited by Sup ODN as well (P<0.01).@*CONCLUSION@#In the presence of pro-Th1-cytokines, Sup ODN may affect the differentiation of CD4(+) T lymphocytes in vitro. Sup ODN can promote CD4(+) T cells to differentiate into Th2, and suppress them into Th1.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligodesoxirribonucleótidos
/
Farmacología
/
Fisiología
/
Linfocitos T CD4-Positivos
/
Diferenciación Celular
/
Subgrupos de Linfocitos T
/
Interferón gamma
/
Relación CD4-CD8
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Células TH1
/
Interleucina-12
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2008
Tipo del documento:
Artículo
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