Preparation and preliminary application of colloidal carbon dipstick for schistosomiasis japonica / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1063-1069, 2009.
Artículo
en Inglés
| WPRIM
| ID: wpr-814192
ABSTRACT
OBJECTIVE@#To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma japonicum (S. japonicum).@*METHODS@#Soluble egg antigen (SEA) of S. japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown.@*RESULTS@#A total of 137 sera samples from S. japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis.@*CONCLUSION@#Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of schistosomiasis japonica. It will be a practical immunological assay for the diagnosis of schistosomiasis in the field testing.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Schistosoma japonicum
/
Sangre
/
Esquistosomiasis Japónica
/
Inmunoensayo
/
Carbono
/
Anticuerpos Antihelmínticos
/
Química
/
Sensibilidad y Especificidad
/
Coloides
/
Diagnóstico
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Journal of Central South University(Medical Sciences)
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS