Effect of hyperglycaemia on fetal heart in pregnant rats / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To investigate the effect of hyperglycaemia on the cardiomyodial change of rat fetus.@*METHODS@#Thirty clean SD pregnant rats were randomly dividing into group A, B and C, 10 in each group. Group A were injected intraperitoneally 50 mg/kg streptozotocin on the 6th day of pregnancy, Group B were injected the same dose on the 13th day of pregnancy, while Group C were injected intraperitoneally 0.1 mmol/L citrate buffer solution on the 6th day of pregnancy. All rats were killed on the 21st day of pregnancy, the total fetus, live fetus, weight, and length of fetus were recorded. The blood glucose in the fetal rats was measured, and the fetal hearts were collected. The fetal hearts were pathologically examined under light microscope and electron microscope. Immunohistochemical staining was applied to determine Caspase-3 in the heart of fetus.@*RESULTS@#(1) The blood glucose of pregnant rats in the 3 groups showed no difference before intervening (P>0.05). There was significant difference between Group A and C, Group B and C after intervening (P0.05). (2 )The fetus in Group A and B was heavier and longer than in Group C, with significant difference (P0.05). The blood glucose of fetus in Group A and B was lower than that in Group C, with significant difference (P0.05). The rate of fetal death in Group A, B, and C were 31.96%,12.84%, and 3.88%, respectively. Significant deviation existed in the 3 groups (P<0.01). (3) Under light microscope, fetal hearts in Group A and B showed disorder, cardiac muscle cells swelled. There were vacuoles in cytolymph and necrosis in the myocardial tissue. Significant deviation in the integral of fetal necrosis existed in the 3 groups (P<0.01). (4) Caspase-3 was detected in the fetal hearts, the positive area ratio and mean OD value had significant deviation in the 3 groups (P<0.01).(5) Under the electron microscope, cardiomyocytes wrinkled, mitochondrion decreased, myofibril ruptured, while sarcomere blurred. The density of mitochondria in cardiamyocyte in Group A was lower than that in Group B and C (P<0.01), and the average volume of mitochondria of Group A and B was higher than that in Group C (P<0.01).@*CONCLUSION@#There is apparent pathological change of fetal hearts in pregnant rats with hyperglycaemia. The longer the duration, the more obvious the change.