Establishment of protein expression profile of laser capture microdissection-purified nasopharyngeal carcinoma tissue / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 481-486, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-814300
ABSTRACT
OBJECTIVE@#To establish the protein expression map of nasopharyngeal carcinoma (NPC), and provide a basis for proteomic study of NPC.@*METHODS@#Laser capture microdissection (LCM) was used to isolate cancer cells from NPC tissues. Two-dimensional gel electrophoresis(2-DE) was used to separate the total proteins of LCM purified NPC cells. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was performed to identify the proteins, and bioinformatics was used to construct the 2-DE database of NPC proteome.@*RESULTS@#A 2-DE reference map of NPC was established. On the 2-DE map, a total of (1 312+/-30) protein spots were detected, and 427 protein spots representing 241 non-redundant proteins were identified. The 2-DE database of NPC proteome was constructed. These data could be accessed at our website (http//www.xyproteomics.org).@*CONCLUSION@#A protein expression profile of LCM purified NPC tissues has been established for the first time, which provides useful information and source for proteomic study of NPC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Nasofaríngeas
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Biología Computacional
/
Proteoma
/
Perfilación de la Expresión Génica
/
Proteómica
/
Microdisección
/
Genética
/
Rayos Láser
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2009
Tipo del documento:
Artículo
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