Effect of formaldehyde on expressions of Fas apoptosis pathway-related genes of ovary tissues in female rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 341-345, 2010.
Article
en Zh
| WPRIM
| ID: wpr-814444
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the mechanism of formaldehyde inducing ovarian toxicity in female rats by observing the effect of formaldehyde on the expression of Fas and caspase-8 mRNA, and the activity of caspase-3 and caspase-8 of ovary tissues in female rats.@*METHODS@#Forty female Sprague-Dawley(SD) rats were randomly divided into a control group and 3 formaldehyde groups at different concentrations. The rats in the formaldehyde groups were intraperitoneally injected different doses of formaldehyde (20.0,2.0 and 0.2 mg/kg) continuously for 14 days.After 14 days, all rats were sacrificed and their ovaries were collected for detecting the expression of Fas and caspase-8 mRNA with RT-PCR, the protein expression of Fas with Western blot, and the activities of caspase-8 and caspase-3 with spectrophotometric method.@*RESULTS@#Compared with the control group, the expression of Fas mRNA and its protein and caspase-8 mRNA and the activity of caspase-8 and caspase-3 of ovary tissues in the rats treated with formaldehyde significantly increased with dose (P<0.05).@*CONCLUSION@#The increase of Fas gene expression and the activity of caspase-8 and caspase-3 may be the important mechanism of ovarian toxicity induced by formaldehyde in female rats.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Ovario
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Patología
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ARN Mensajero
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Distribución Aleatoria
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Ratas Sprague-Dawley
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Apoptosis
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Receptor fas
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Contaminantes Ambientales
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Toxicidad
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Caspasa 3
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2010
Tipo del documento:
Article