Proteomics of the colonic mucosa in sub-healthy people with shapeless stool / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 817-822, 2011.
Article
en Zh
| WPRIM
| ID: wpr-814495
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish the 2-dimensional electrophoresis (2-DE) map in colonic mucosa in sub-healthy people with shapeless stool and healthy people, to identify the differential proteins by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and to provide theoretical basis for the pathogenesis of intestinal mucosa in sub-healthy people with shapeless stool.@*METHODS@#Two-DE was used to separate the total proteins from the intestinal mucosa in sub-healthy people (the sub-health group) with the shapeless stool and healthy volunteers (the control group). ImageMaster 2D Elite soft was applied to analyze the 2-DE images, and the differentially expressed protein spots between the 2 groups were identified by MALDI-TOF-MS, protein bank and information technique.@*RESULTS@#We analyzed the average maps and obtained 517 protein spots in the sub-healthy group and 535 protein spots in the control group. Between the sub-healthy group and the control group, the mean of 366 protein spots was matched, and the matching rate was 70.79%. Ten differential protein spots were screened by MALDI-TOF-MS, and 8 were identified. Five out of the 8 spots were significantly decreased, while 3 out of the 8 were significantly increased.@*CONCLUSION@#The proteomic expression in colonic mucosa of people with shapeless stool is significantly different from that of healthy people. Eight differential proteins such as aldehyde dehydrogenase 1A1 isoform 1, 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (mitochondrial), γ-actin, annexin A5 possibly involve in the pathogenesis of sub-healthy people with shapeless stool.
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Índice:
WPRIM
Asunto principal:
Electroforesis en Gel Bidimensional
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Proteínas
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Estudios de Casos y Controles
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Actinas
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Anexina A5
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Colon
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Proteoma
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Proteómica
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Aldehído Deshidrogenasa
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Dispepsia
Tipo de estudio:
Observational_studies
Límite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Central South University(Medical Sciences)
Año:
2011
Tipo del documento:
Article