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miR-181a promotes the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 789-795, 2016.
Artículo en Chino | WPRIM | ID: wpr-814962
ABSTRACT
OBJECTIVE@#To investigate the role of miR-181a in promoting the proliferation and metastasis of osteosarcoma cells by targeting RASSF1A.
@*METHODS@#The level of miR-181a in 30 human osteosarcoma tissues and corresponding bone tissues was detected by real-time PCR, and the correlation between the level of miR-181a and clinicopathological characteristics of osteosarcoma was analyzed. Osteosarcoma cells MG-63 were transfected with chemically-synthesized miR-181a mimics and inhibitors, and the proliferation, migration and invasion of MG-63 cells were detected by MTT and Transwell assay. The specific binding ability of miR-181a to RASSF1A 3'-UTR was theoretically predicted and detected by the dual luciferase reporter gene assay.
@*RESULTS@#The level of miR-181a in osteosarcoma tissues was statistically higher than that in the corresponding bone tissues (P0.05). MTT and Transwell assays showed that the growth rate, migration and invasion ability of MG-63 cells with up-regulation of miR-181a was significantly increased compared with negative control (P<0.05), while the growth rate, migration and invasion ability of MG-63 with down-regulated miR-181a was significantly decreased compared with negative control (P<0.05). Luciferase reporter gene assay showed that miR-181a targeted the 3'-UTR of RASSF1A and regulated the expression of RASSF1A.
@*CONCLUSION@#MiR-181a promotes the proliferation and metastasis of osteosarcoma cells through specifically binding to RASSF1A 3'-UTR and subsequent down-regulation of RASSF1A.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Transfección / Osteosarcoma / Regulación hacia Abajo / Regulación hacia Arriba / Proteínas Supresoras de Tumor / MicroARNs / Línea Celular Tumoral / Proliferación Celular / Reacción en Cadena en Tiempo Real de la Polimerasa / Metástasis de la Neoplasia Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Chino Revista: Journal of Central South University(Medical Sciences) Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Transfección / Osteosarcoma / Regulación hacia Abajo / Regulación hacia Arriba / Proteínas Supresoras de Tumor / MicroARNs / Línea Celular Tumoral / Proliferación Celular / Reacción en Cadena en Tiempo Real de la Polimerasa / Metástasis de la Neoplasia Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Chino Revista: Journal of Central South University(Medical Sciences) Año: 2016 Tipo del documento: Artículo