Effect of calcium channel blockers on primary cultured human urethra scar fibroblasts / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1317-1322, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-815091
ABSTRACT
To investigate effects of verapamil on primary cultured human urethral scar fibroblasts (USFs) and to provide basis for protecting the formation of urethra scar.
Methods:
The cell proliferation was evaluated with the cell counting kit (CCK)-8 method after USFs were incubated various verapamil concentrations (50, 100, 150, 200, or 250 μmol/L) or solvent for 12, 24, or 48 h. The protein level of matrix metalloproteinase (MMP) was evaluated with ELISA after cells were incubated with verapamil (100 μmol/L) or solvent (control cells) for 24 h.Results:
The proliferation of USFs was obviously suppressed after verapamil treatment, which was in a dose-dependent and time-dependent manner. Meanwhile, the protein levels of MMP-2 and MMP-9 in the verapamil treatment group increased obviously compared with those of the control groups (P<0.05).Conclusion:
Calcium channel blockers may prevent the excessive formation of urethra scar by inhibiting the proliferation of urethral scar fibroblasts and enhancing the activity of MMP.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Uretra
/
Bloqueadores de los Canales de Calcio
/
Verapamilo
/
Regulación hacia Arriba
/
Células Cultivadas
/
Cicatriz
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Central South University(Medical Sciences)
Año:
2016
Tipo del documento:
Artículo
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