L10P mutation in DJ-1 gene induced oxidative stress and mitochondrial disfunction / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1285-1291, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-815339
ABSTRACT
OBJECTIVE@#To investigate the effect of the L10P mutation on the cellular mitochondrial disfunction.@*METHODS@#Spectrophotometer, flow cytometry and electron microscope was utilized to examine cell viability, reactive oxygen species (ROS), mitochondrial transmembrane potential, complex I activity and mitochondrial morphous of the HEK293 monoclone cell lines, in which wild-type and L10P mutant DJ-1 protein are stably expressed.@*RESULTS@#Compared with the cell lines expressing empty vector, we found the ROS levels were elevated, the cell viability, mitochondrial transmembrane potential, complex I activity were reduced in the cells expressing L10P mutant DJ-1 protein (P<0.05). We also found mitochondria in these cells were swelling and some mitochondria were vacuolar degeneration. These phenomena were more obvious when rotenone was used. But in the cells expressing wild-type DJ-1, ROS levels were lower, the cell viability, mitochondrial transmembrane potential, and complex I activity were higher than other cell lines (P<0.05), especially under the induction of rotenone. These results suggested that L10P mutant DJ-1 protein probably lost the ability of anti-oxidative stress and affect the normal function of mitochondria.@*CONCLUSION@#The L10P DJ-1 mutation results in a toxic protein, which lacks the protective function of wild-type protein on mitochondria due to the decrease in the ability of anti-oxidative stress.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Rotenona
/
Supervivencia Celular
/
Especies Reactivas de Oxígeno
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Proteínas Oncogénicas
/
Estrés Oxidativo
/
Péptidos y Proteínas de Señalización Intracelular
/
Potencial de la Membrana Mitocondrial
/
Células HEK293
/
Proteína Desglicasa DJ-1
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Central South University(Medical Sciences)
Año:
2015
Tipo del documento:
Artículo
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