Optimization of Extraction Technology of Osthole from Pine Needles of Cedrus deodara by Box-Behnken Design-response Surface Methodology / 中国药房
China Pharmacy
;
(12): 359-363, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-816889
ABSTRACT
OBJECTIVE: To optimize the extraction technology of osthole from the pine needles of Cedrus deodara. METHODS: HPLC method was adopted to determine the content of osthole. Based on single factor test, ethanol volume fraction, extraction time and material-solvent ratio were selected as influential factors, and the content of osthole was selected as response value. Box-Behnken design-response surface methodology was used to optimize the extraction technology of osthole in pine needles of C. deodara. Validation test was conducted. RESULTS: The optimal extraction technology was as follows as ethanol volume fraction of 88%, material-solvent ratio of 1 ∶ 20 (g/mL), extracting for 2 times, lasting for 57 min each time. Under this technology, average content of osthole was 0.675 7 mg/g (RSD=1.78%, n=3), and the relative error of which to predicted value 0.680 9 mg/g was 0.59%. CONCLUSIONS: The optimal extraction technology is simple and feasible,and it can be used for the extraction of osthole from the pine needles of C. deodara.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
China Pharmacy
Año:
2019
Tipo del documento:
Artículo
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