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Comparison of Low-polarity Volatile Constituents in Supercritical CO2 Extract from the Roots and Stem of Ilex asprella and Its Effects on the Proliferation of IEC- 6 Cells / 中国药房
China Pharmacy ; (12): 1056-1060, 2019.
Artículo en Chino | WPRIM | ID: wpr-816989
ABSTRACT
OBJECTIVE: To compare low-polarity volatile constituents in supercritical CO2 extract from the roots and stem of Ilex asprella and its effects on the proliferation of IEC-6 in vitro, and to provide reference for making full use of wild resources of I. asprella and expanding its medicinal parts. METHODS: The low-polarity volatile constituents were extracted from the root and stem of I. asprella with supercritical fluid CO2 extraction(SFE-CO2). The chemical constituents were analyzed by GC-MS. IEC-6 cells were treated with different concentrations of supercritical CO2 extracts (0, 1, 5, 10, 20, 40, 60, 80, 100 μg/mL) from roots or stems of I. asprella. MTT assay was used to detect the relative viability, and cell proliferation curve was drawn and EC50 of each extract were calculated. RESULTS: Sixty-two and forty-six low-polarity volatile constituents were identified from supercritical CO2 extract in the roots and stem of I. asprella with GC-MS; there were 24 common constituents totally, mainly including pelargonic acid(14.18% and 6.14%),octanoic acid(10.59% and 4.35%),hexanoic acid(8.63% and 10.86%),paeonol(7.79% and 6.00%),2-methyl-3-phenyl-propanal(6.3% and 0.58%),acetic acid(1.72% and 33.77%) in root and stem, respectively. The results of cell culture in vitro showed that when the concentration of supercritical CO2 extract from the roots and stems of I. asprella was lower (≤60 μg/mL), it could significantly promote the proliferation of IEC-6 cells and their EC50 were 16.35, 20.20 μg/mL, respectively; when the concentration of the extract was higher (≥80 μg/mL), it showed cytotoxicity and inhibited the proliferation of IEC-6 cells. CONCLUSIONS: There are similar species of volatile constituents in roots and stems of I. asprella and similar in vitro bioactivity of the supercritical CO2 extracts to IEC-6 cells. The short-chain fatty acids may be the active ingredient to promote cell proliferation, while paeonol may be the cytotoxic active ingredient.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: China Pharmacy Año: 2019 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: China Pharmacy Año: 2019 Tipo del documento: Artículo