Isolation and Identification the Compositions of Alkaloids in Acorus tatarinowii / 中国药房

ABSTRACT

OBJECTIVE： To isolate and identify the compositions of alkaloids in Acorus tatarinowii， and to lay the foundation for further study of its pharmacological activity. METHODS： The compositions of alkaloids from A. tatarinowii was separated by cation exchange resin. UPLC-MS method was used to identify the alkaloids. The separation was performed on a Agilent Zorbax Eclipse Plus-C18 column with mobile phase consisted of 0.1％ formic acid-acetonitrile （gradient elution） at a flow rate of 0.3      mL/min. The column temperature was 30 ℃， and the injection volume was 1 μL. ESI ion source was used to detect the fragments， the ion mode scan was positive. Peakview 1.2 and other softwares were used to analyze the compositions of alkaloids. RESULTS： 86 compositions were separated and 5 alkaloids were identified， such as tatarine A， N-trans-feruloyltyramine， N-lauryldiethanolamine， phytosphingosine and 5-butyluridine. CONCLUSIONS： The results of the study lay a foundation for the study of pharmacological activity of alkaloids from A. tatarinowii.