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Simultaneous Determination of 8 Non-anthraquinone Constituents in Rheum palmatum by HPLC / 中国药房
China Pharmacy ; (12): 1975-1980, 2019.
Artículo en Chino | WPRIM | ID: wpr-817217
ABSTRACT
OBJECTIVE: To establish a method for simultaneous determination of 8 non-anthraquinone constituents in Rheum palmatum. METHODSHPLC method was adopted. The determination was performed on Symmetry C18 column with mobile phase consisted of methanol-0.1% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The column temperature was 30 ℃ and detection wavelength was 280 nm. Sample size was 30 μL. RESULTS: The linear range of gallic acidcatechinepicatechinresveratrol 4′-O-glucopyranoside, epicatechin gallate, resveratrol 4′-O-β-D-(6″-O-galloyl)-glucopyranoside, sennoside A, 4′-hydroxyphenyl-2-butanone-4′-O-β-D-(2″-O-galloyl-6″-O-p-hydroxy cinnamyl)-glucopyranoside were 6.16-2 464 ng(r=0.999 9), 37.4-14 960 ng(r=0.999 9), 7.635-3 054 ng(r=0.999 7), 7.63-3 052 ng(r=0.999 9), 8.32-3 328 ng(r=0.999 9), 11.5-4 600 ng(r=0.999 9), 16.08-6 432 ng(r=0.999 9), 29.3-11 720 ng(r=0.999 9), respectively. The limits of quantitation were 3.48, 4.30, 6.40, 4.40, 3.39, 2.87, 8.40 and 4.95 ng, respectively. The limits of detection were 2.32, 2.58, 2.40, 2.64, 2.26, 1.23, 4.20, 2.97 ng, respectively. RSDs of precision, stability and reproducibility tests were all lower than 5%. Recoveries were 94.32%- 100.54%(RSD=2.78%,n=6), 91.15%-99.36%(RSD=3.72%,n=6), 92.16%-98.04%(RSD=2.39%,n=6), 93.41%-100.73%(RSD=3.17%,n=6), 93.89%-98.40%(RSD=1.99%,n=6), 92.61%-101.74%(RSD=3.71%,n=6), 92.66%-103.40%(RSD=3.76%,n=6), 95.45%-102.70%(RSD=3.06%,n=6), respectively. CONCLUSIONS: The established method is simple, accurate and specific, and can be used for the simultaneous determination of 8 non-anthraquinone constituents in R. palmatum.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: China Pharmacy Año: 2019 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: China Pharmacy Año: 2019 Tipo del documento: Artículo