Effects of Processed Polygonum multiflorum Containing Serum on the Proliferation and the Expression of ER of Human Breast Cancer T- 47D Cells / 中国药房
China Pharmacy
; (12): 3062-3067, 2019.
Article
en Zh
| WPRIM
| ID: wpr-817442
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WPRO
ABSTRACT
OBJECTIVE: To study the effects of processed Polygonum multiflorum containing serum on the proliferation and the expression of estrogen receptor (ER) of human breast cancer T-47D cells, and to investigate its phytoestrogen (PE)-like effect. METHODS: Sexually immature SD rats were randomly divided into estradiol valerate (Ev) group (positive control, 0.12 mg/kg), processed P. multiflorum low-dose and high-dose groups (0.75, 3 g/kg, by crude drug), low-dose and high-dose processed P. multiflorum+Ev groups (same dose as single drug group), with 10 rats in each group. Blank group was given constant volume of water intragastrically, and administration groups were given relevant medicine intragastrically; once day and night, for consecutive 4 days. Two hours after last administration, blank serum and containing serum were prepared. T-47D cells were also randomly divided into blank group, Ev group, low-dose and high-dose processed P. multiflorum groups, low-dose and high-dose processed P. multiflorum+Ev groups, and then were cultured in medium which contained 20% blank serum or drug containing serum. CCK-8 assay was used to detect proliferation rate (PR). Western blotting assay and RT-PCR were used to detect the protein and mRNA expression of ER-α and ER-β. RESULTS: Compared with blank group, PR of administration groups [each administration group (24 h), other administration groups (48, 72 h) except for high-dose processed P. multiflorum+Ev group] were increased significantly; high-dose processed P. multiflorum group (72 h) was significantly higher than Ev group, and low-dose processed P. multiflorum+Ev group (72 h) was significantly higher than the same-dose processed P. multiflorum group; high-dose processed P. multiflorum+Ev group (72 h) was significantly lower than the same-dose processed P. multiflorum group (P<0.05 or P<0.01). Relative protein expression of ER-α in Ev group, high-dose processed P. multiflorum group and low-dose processed P. multiflorum+Ev group, relative mRNA expression of ER-α and protein expression of ER-β in administration groups, relative mRNA expression of ER-β in Ev group, low-dose processed P. multiflorum group and processed P. multiflorum+Ev groups were all increased significantly. Relative protein and mRNA expression of ER-α in Ev group were significantly higher than processed P. multiflorum groups and combination groups. Relative protein and mRNA expression of ER-β in Ev group were significantly lower than low-dose processed P. multiflorum+Ev group, but relative mRNA expression of ER-β was significantly higher than processed P. multiflorum groups and high-dose processed P. multiflorum+Ev group. Relative protein and mRNA expression of ER-α and ER-β in low-dose processed P. multiflorum+Ev group as well as relative mRNA expression of ER-β in high-dose processed P. multiflorum+Ev group were significantly higher than the same-dose processed P. multiflorum group. Relative protein and mRNA expression of ER-α in high-dose processed P. multiflorum+Ev group were significantly lower than the same-dose processed P. multiflorum group (P<0.05 or P<0.01). CONCLUSIONS: The processed P. multiflorum containing serum can promote the proliferation of human breast cancer T-47D cells, and play the PE-like role through promoting protein and mRNA expression of ER-α and ER-β. However, the above effects are weaker than estrogen, and the combination of the two may antagonize the effect of estrogen.
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WPRIM
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Zh
Revista:
China Pharmacy
Año:
2019
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Article