Icaritin promotes maturation and mineralization of mouse osteoblast MC3T3-E1 cells through CXCR4/SDF-1 signal pathway / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 571-577, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-819079
ABSTRACT
Objective:
To investigate the effect of icaritin on maturation and mineralization of mouse osteoblast MC3T3-E1 cells and its mechanism.Methods:
The cultured MC3T3-E1 cells were divided into blank control group, CXC chemokine receptor type 4 (CXCR4) inhibitor (AMD3100) group, icaritin group, and icaritin plus AMD3100 group. The expression of CXCR4, stromal cell-derived factor 1 (SDF-1) and osteogenesis-related genes and proteins were detected by real-time RT-PCR and Western blotting after drug treatment for 24 h. The alkaline phosphatase (ALP) activity was determined with ALP kit on d3 and d6; calcium nodules were detected by alizarin red staining after drug treatment for 14 d.Results:
Real time RT-PCR showed that compared with the blank control group, relative expressions of CXCR4, SDF-1 and osteogenesis-related genes in icaritin group were significantly increased (PPCXCR4 gene was decreased (PPPPPConclusion:
Icaritin may promote maturation and mineralization of mouse osteoblast MC3T3-E1 cells through CXCR4/SDF-1 signaling pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Farmacología
/
Flavonoides
/
Calcificación Fisiológica
/
Transducción de Señal
/
Células 3T3
/
Regulación del Desarrollo de la Expresión Génica
/
Receptores CXCR4
/
Biología Celular
/
Quimiocina CXCL12
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2017
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS