Screening of differentially expressed microRNA in ulcerative colitis related colorectal cancer
Asian Pacific Journal of Tropical Medicine
;
(12): 972-976, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-819747
ABSTRACT
OBJECTIVE@#To investigate the differential expression of microRNA (miRNA) in colon between ulcerative colitis (UC) and ulcerative colitis related colorectal cancer (UCRCC).@*METHODS@#An UC mouse model was built by dextran sodium sulfate, and an UCRCC mouse model by dextran sodium sulfate and 1,2-diformylhydrazine. RNAs were extracted from the colon, purified and hybridized with fluorescence-labeled miRNA oligonucleotide gene chip. Real-time fluorescence quantitative PCR was used to verify the expression variation of miRNA. SAM was employed for the data analysis.@*RESULTS@#The up-regulated miRNAs in colon cancer included has-miR-194, has-miR-215, has-miR-93, has-miR-192, has-miR-92a, has-miR-29b, and has-miR-20a (median false discovery rate<5%), while the down-regulated miRNAs were has-miR-1231, has-miR-195, has-miR-143, and has-miR-145 (median false discovery rate<5%).@*CONCLUSIONS@#Significant differential expression of miRNA was found between the UC mouse and UCRCC mouse, which may be related to the onset, erosion and transfer of colorectal cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Colorrectales
/
Colitis Ulcerosa
/
Regulación hacia Abajo
/
Regulación Neoplásica de la Expresión Génica
/
Regulación hacia Arriba
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
MicroARNs
/
Modelos Animales de Enfermedad
/
Genética
/
Metabolismo
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Inglés
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2013
Tipo del documento:
Artículo
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