Effect of ERBB2 expression on invasiveness of glioma TJ905 cells
Asian Pacific Journal of Tropical Medicine
;
(12): 964-967, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-819749
ABSTRACT
OBJECTIVE@#To investigate the influence and possible mechanism of ERBB2 expression on the invasiveness of glioma cells.@*METHODS@#Glioma TJ905 cells were separated and cultured. ERBB2 shRNA and overexpressing vectors were constructed, which were then transfected. The ERBB2 expression was up-regulated or down-regulated. Changes of invasiveness of TJ905 cells were detected by Transwell assay, and the expressions of matrix metalloprotease (MMP)-2 and MMP-9 were measured by Western blot.@*RESULTS@#ERBB2 shRNA transfection vector could effectively inhibit expression of ERBB2; while ERBB2 overexpressing vector transfection could significantly improve the expression of ERBB2 in TJ905 cells. Transwell assay showed that when ERBB2 expression was down-regulated, the invasiveness of TJ905 cells was notably decreased; when ERBB2 expression was up-regulated, the invasiveness of TJ905 cells was markedly increased. Meanwhile, Western blot indicated that down-regulating ERBB2 inhibited the expression of MMP-2 and MMP-9, while up-regulating ERBB2 enhanced their expressions.@*CONCLUSIONS@#ERBB2 expression is closely related to the invasiveness of glioma TJ905 cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Regulación Neoplásica de la Expresión Génica
/
Regulación hacia Arriba
/
Receptor ErbB-2
/
ARN Interferente Pequeño
/
Línea Celular Tumoral
/
Genética
/
Glioma
/
Metabolismo
/
Invasividad Neoplásica
Límite:
Humanos
Idioma:
Inglés
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2013
Tipo del documento:
Artículo
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