Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus
Asian Pacific Journal of Tropical Medicine
;
(12): 342-346, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-819771
ABSTRACT
OBJECTIVE@#To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.@*METHODS@#We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.@*RESULTS@#The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.@*CONCLUSIONS@#This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sondas de ADN
/
Virus Chikungunya
/
Sensibilidad y Especificidad
/
Infecciones por Alphavirus
/
Técnicas de Amplificación de Ácido Nucleico
/
Dengue
/
Virus del Dengue
/
Diagnóstico
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Fiebre Chikungunya
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2012
Tipo del documento:
Artículo
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