Effects of arteannuin B, arteannuic acid and scopoletin on pharmacokinetics of artemisinin in mice

ABSTRACT

OBJECTIVE@#To explore the effects of arteannuin B, arteannuic acid and scopoletin on the pharmacokinetics of artemisinin in mice.@*METHODS@#Artemisinin and a combination of artemisinin, arteannuin B, arteannuic acid and scopoletin were administered together to mice via oral administration. Blood samples were collected at different time intervals and pretreated by liquid-liquid extraction. The contents of four compounds in mouse plasma were determined by a validated HPLC-MS/MS method.@*RESULTS@#Compared to single artemisinin group, the Cmax values from the combination group rose from 947 ng/mL to 1254 ng/mL. AUC(0-t) (2371 h ng/mL) was significantly higher than that from single artemisinin group (747 h ng/mL). The peak time lag and the CL values reduced at a proportion of 66%.@*CONCLUTIONS@#Arteannuin B, arteannuic acid and scopoletin can markedly affect the pharmacokinetics of artemisinin.