Identification of target genes of transcription factor CEBPB in acute promyelocytic leukemia cells induced by all-trans retinoic acid
Asian Pacific Journal of Tropical Medicine
;
(12): 473-480, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-820018
ABSTRACT
OBJECTIVE@#To identify target genes of transcription factor CCAAT enhancer-binding protein β (CEBPB) in acute promyelocytic leukemia cells induced by all-trans retinoic acid.@*METHODS@#A new strategy for high-throughput identification of direct target genes was established by combining chromatin immunoprecipitation (ChIP) with in vitro selection. Then, 106 potential CEBPB binding fragments from the genome of the all-trans retinoic acid (ATRA)-treated NB4 cells were identified.@*RESULTS@#Of them, 82 were mapped in proximity to known or previously predicted genes; 7 were randomly picked up for further confirmation by ChIP-PCR and 3 genes (GALM, ITPR2 and ORM2) were found to be specifically up-regulated in the ATRA-treated NB4 cells, indicating that they might be the down-stream target genes of ATRA.@*CONCLUSIONS@#Our results provided new insight into the mechanisms of ATRA-induced granulocytic differentiation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Tretinoina
/
Sitios de Unión
/
Leucemia Promielocítica Aguda
/
Transducción de Señal
/
Regulación Neoplásica de la Expresión Génica
/
Diferenciación Celular
/
Línea Celular
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS