Your browser doesn't support javascript.
loading
Nevirapine induces apoptosis in liver (HepG2) cells
Asian Pacific Journal of Tropical Medicine ; (12): 547-553, 2016.
Artículo en Inglés | WPRIM | ID: wpr-820228
ABSTRACT
OBJECTIVE@#To generate insights into the mechanism of NVP induced hepatotoxicity.@*METHODS@#Liver (HepG2) cells were cultured with various concentrations of NVP. This cell line was chosen because it has low expression of cytochrome P450, allowing evaluation of the effects of NVP rather than specific metabolites. Cytotoxicity was determined using a proliferation assay and cell numbers were monitored using trypan blue exclusion assay for long term culture experiments and apoptosis induction was determined by morphological and biochemical investigation.@*RESULTS@#HepG2 cells treated with the highest concentration of NVP tested (819 μM) initially showed a rounded morphology and all cells had died by week three of exposure. Nuclear condensation and fragmentation, increased Annexin V/propidium iodide staining and caspase 9 activation all supported the induction of apoptosis in HepG2 cells in response to NVP treatment.@*CONCLUSIONS@#There is a clear induction of apoptosis in response to NVP which suggests that NVP has significant cytotoxicity, over and above any cytotoxicity of metabolites and may contribute directly to patient hepatotoxicity.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Inglés Revista: Asian Pacific Journal of Tropical Medicine Año: 2016 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Inglés Revista: Asian Pacific Journal of Tropical Medicine Año: 2016 Tipo del documento: Artículo