Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae
Asian Pacific Journal of Tropical Medicine
;
(12): 115-118, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-820558
ABSTRACT
OBJECTIVE@#To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.@*METHODS@#Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.@*RESULTS@#According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.@*CONCLUSIONS@#Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Represoras
/
Proteínas Bacterianas
/
Vibrio cholerae
/
Virulencia
/
ADN Bacteriano
/
Cólera
/
Sensibilidad y Especificidad
/
Clasificación
/
Heces
/
Reacción en Cadena de la Polimerasa Multiplex
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2013
Tipo del documento:
Artículo
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