In vitro study on blocking mTOR signaling pathway in EGFR-TKI resistance NSCLC
Asian Pacific Journal of Tropical Medicine
; (12): 394-397, 2014.
Article
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| ID: wpr-820698
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ABSTRACT
OBJECTIVE@#To investigate the effect and mechanism of inhibitor everolimus on EGFR-TKI resistance NSCLC.@*METHODS@#MTT assay was used to detect proliferation of human non-small cell lung cancer cell line A549. Flow cytometry was used to detect the changes of apoptosis and cycle distribution in each group after 24 h and 48 h. RT-PCR was used to detect the changes of PTEN and 4EBP1 expression levels after 48 h of monotherapy and combination therapy.@*RESULTS@#MTT assay showed that everolimus had dose-dependent inhibition against growth of A549 cells. Flow cytometry showed when everolimus could induce apoptosis and induce G0/G1 phase cell cycle arrest, which was time-dependent (P<0.05). RT-PCR showed everolimus could increase PTEN and 4EBP1 expression.@*CONCLUSIONS@#mTOR inhibitor everolimus has an inhibitory effect on EGFR-TKI resistant NSCLC, which cannot reverse the resistance effect of EGFR-TKI resistant cell line A549. The relationship between EGFR/AKT signaling pathway and the mTOR signaling pathway and the mechanism in non-small cell lung cancer need further study.
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Asunto principal:
Farmacología
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Fosfoproteínas
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Transducción de Señal
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Regulación Neoplásica de la Expresión Génica
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Apoptosis
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Carcinoma de Pulmón de Células no Pequeñas
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Sirolimus
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Línea Celular Tumoral
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Proteínas Adaptadoras Transductoras de Señales
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Proliferación Celular
Límite:
Humans
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2014
Tipo del documento:
Article