FKBP-12 Exhibits an Inhibitory Activity on Calcium Oxalate Crystal Growth in Vitro
Journal of Korean Medical Science
; : 41-48, 2002.
Article
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| ID: wpr-82627
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WPRO
ABSTRACT
Urolithiasis and calcium oxalate crystal deposition diseases are still significant medical problems. In the course of nephrocalcin cDNA cloning, we have identified FKBP-12 as an inhibitory molecule of calcium oxalate crystal growth. lambdagt 11 cDNA libraries were constructed from renal carcinoma tissues and screened for nephrocalcin cDNA clones using anti-nephrocalcin antibody as a probe. Clones expressing recombinant proteins, which appeared to be antigenically cross-reactive to nephrocalcin, were isolated and their DNA sequences and inhibitory activities on the calcium oxalate crystal growth were determined. One of the clone lambdagt 11 #31-1 had a partial fragment (80 bp) of FKBP-12 cDNA as an insert. Therefore, a full-length FKBP-12 cDNA was PCR-cloned from the lambdagt 11 renal carcinoma cDNA library and was subcloned into an expression vector. The resultant recombinant FKBP-12 exhibited an inhibitory activity on the calcium oxalate crystal growth (Kd=10(-7) M). Physiological effect of the extracellular FKBP-12 was investigated in terms of macrophage activation and proinflammatory cytokine gene induction. Extracellular FKBP-12 failed to activate macrophages even at high concentrations. FKBP-12 seems an anti-stone molecule for the oxalate crystal deposition disease and recurrent stone diseases.
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WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
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Oxalato de Calcio
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Datos de Secuencia Molecular
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Secuencia de Bases
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Glicoproteínas
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Carcinoma de Células Renales
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Cálculos Renales
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ADN Complementario
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Cristalización
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Proteína 1A de Unión a Tacrolimus
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Journal of Korean Medical Science
Año:
2002
Tipo del documento:
Article