Numerical simulation and optimization of impeller combination used in stirred bioreactor / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1209-1215, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-826857
ABSTRACT
Bioreactors have been central in monoclonal antibodies and vaccines manufacturing by mammalian cells in suspension culture. Numerical simulation of five impeller combinations in a stirred bioreactor was conducted, and characteristics of velocity vectors, distributions of gas hold-up, distributions of shear rate in the bioreactor using 5 impeller combinations were numerically elucidated. In addition, genetically engineered CHO cells were cultivated in bioreactor installed with 5 different impeller combinations in fed-batch culture mode. The cell growth and antibody level were directly related to the maximum shear rate in the bioreactor, and the highest viable cell density and the peak antibody level were achieved in FBMI3 impeller combination, indicating that CHO cells are sensitive to shear force produced by impeller movement when cells were cultivated in bioreactor at large scale, and the maximum shear rate would play key roles in scaling-up of bioreactor at industrial scale.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estándares de Referencia
/
Simulación por Computador
/
Microbiología Industrial
/
Recuento de Células
/
Cricetulus
/
Células CHO
/
Reactores Biológicos
/
Técnicas de Cultivo Celular por Lotes
/
Métodos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2020
Tipo del documento:
Artículo
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