Cloning, expression and characterization of a new endo-β-N-acetylglucosaminidase from Streptomyces alfalfae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 932-941, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-826883
ABSTRACT
Endo-β-N-acetylglucosaminidase is used widely in the glycobiology studies and industries. In this study, a new endo-β-N-acetylglucosaminidase, designated as Endo SA, was cloned from Streptomyces alfalfae ACCC 40021 and expressed in Escherichia coli BL21 (DE3). The purified recombinant Endo SA exhibited the maximum activity at 35 ºC and pH 6.0, good thermo/pH stability and high specific activity (1.0×10⁶ U/mg). It displayed deglycosylation activity towards different protein substrates. These good properties make EndoSA a potential tool enzyme and industrial biocatalyst.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Streptomyces
/
Estabilidad de Enzimas
/
Proteínas Recombinantes
/
Expresión Génica
/
Manosil-Glicoproteína Endo-beta-N-Acetilglucosaminidasa
/
Clonación Molecular
/
Escherichia coli
/
Genética
/
Metabolismo
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2020
Tipo del documento:
Artículo
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