PDGFRα Cell-Derived SCF Regulates Hematopoiesis of Adult Mice / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1349-1356, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-827113
ABSTRACT
OBJECTIVE@#To investigate the effect of PDGFRα stromal cells derived SCF on hematopoiesis of adult mice.@*METHODS@#Pdgfrα-CreER; R26-tdTomato mice model was constructed, and the proportion and distribution of PDGFRα cells in the liver, spleen, lung, kidney and bone marrow were analyzed by flow cytometry and confocal microscopy. Then the Pdgfrα-CreER; Scf mice model was further constructed, the Scf in PDGFRα was knocked out specifically, the effect of Scf-knocked out in PDGFRα stromal cells in the propitiation of HSPCs in the bone marrow was analyzed by flow cytometry. The effect of SCF on the proportion on number of peripheral blood cells in mice was analyzed by whole blood analyzer.@*RESULTS@#After Scf was knocked out in PDGFRα stromal cells, the propitiation and number of LKS- cell, LKS+ cell, HSC, MPP1, MKP, PreGM, PreMegE, and CFU-E in the bone marrow of mice was decreased, as well as in the number of red blood cells and hemoglobin concentration of peripheral blood. However, Scf knocked out from PDGFRα cells showed no effect on the hematopoiesis in spleen.@*CONCLUSION@#specific knocked out of Scf in PDGFRα stromal cells in adult mice can decrease the proportion of HSPCs in the bone marrow and the number of red blood cells in peripheral blood, and finally lead to anemia in mice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Médula Ósea
/
Células de la Médula Ósea
/
Factor de Células Madre
/
Receptor alfa de Factor de Crecimiento Derivado de Plaquetas
/
Hematopoyesis
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2020
Tipo del documento:
Artículo
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