A Report of Cat Scratch Disease in Korea Confirmed by PCR Amplification of the 16S-23S rRNA Intergenic Region of Bartonella henselae / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 34-37, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-82771
ABSTRACT
We report a case of cat scratch disease in an 8-yr-old girl who presented with fever and enlargement of both axillary lymph nodes. Both aerobic and anaerobic cultures of the lymph node aspirate were negative for microbial growth. Gram staining and Warthin-Starry silver staining did not reveal any organism. Purified DNA from the PCR-amplicon of the 16S-23S rRNA intergenic region was sequenced and showed 99.7% identity with the corresponding sequence of Bartonella henselae strain Houston-1. Our findings suggest that the internal transcribed spacer is a reliable region for PCR identification of Bartonella species. In patients with lymphadenitis, a history of contact with cats or dogs necessitates the use of diagnostic approaches that employ not only the conventional staining and culture but also molecular methods to detect B. henselae.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Ribosómico 16S
/
ARN Ribosómico 23S
/
Enfermedad por Rasguño de Gato
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Bartonella henselae
/
República de Corea
/
Linfadenitis
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Femenino
/
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
The Korean Journal of Laboratory Medicine
Año:
2010
Tipo del documento:
Artículo
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