Establishment of a vimentin knockout and HIV-1 gp120 transgenic mouse model / 南方医科大学学报
Journal of Southern Medical University
;
(12): 519-524, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-828105
ABSTRACT
OBJECTIVE@#To construct a HIV-1 gp120 transgenic mice (gp120 Tg) with vimentin (VIM) gene knockout.@*METHODS@#Female HIV-1 gp120 Tg mice were mated to VIM heterozygote mice (F0). All the offspring mice were derived from these original founders so that both genotypes had the same mixed genetic background. The F1 mice were bred to generate of VIM, VIM, VIM/gp120 Tg and VIM/gp120 Tg mice. PCR was performed for genotyping of the mice, and the expressions of VIM and gp120 in the brain tissues were examined using immunoblotting.@*RESULTS@#The results of PCR showed the presence of the target bands in VIM, VIM, VIM/gp120 Tg and VIM/gp120 Tg mice. In VIM/gp120 Tg mice, gp120 expression was detected throughout the brain regions while no VIM expression was detected.@*CONCLUSIONS@#We generated gp120 transgenic mouse models with VIM gene knockout, which facilitate the exploration of the role of VIM in gp120-induced neurotoxicity.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Vimentina
/
Encéfalo
/
Ratones Transgénicos
/
Proteína gp120 de Envoltorio del VIH
/
VIH-1
/
Ratones Noqueados
/
Modelos Animales de Enfermedad
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2020
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS