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Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation / 国际口腔科学杂志·英文版
International Journal of Oral Science ; (4): 18-18, 2020.
Artículo en Inglés | WPRIM | ID: wpr-828960
ABSTRACT
Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteogénesis / Periodontitis Periapical / Farmacología / Células Madre / Terapéutica / Berberina / Diferenciación Celular / Biología Celular / Papila Dental / Microtomografía por Rayos X Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: International Journal of Oral Science Año: 2020 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Osteogénesis / Periodontitis Periapical / Farmacología / Células Madre / Terapéutica / Berberina / Diferenciación Celular / Biología Celular / Papila Dental / Microtomografía por Rayos X Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: International Journal of Oral Science Año: 2020 Tipo del documento: Artículo