Characteristics of oral methicillin-resistant Staphylococcus epidermidis isolated from dental plaque / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 15-15, 2020.
Article
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| ID: wpr-828963
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WPRO
ABSTRACT
The oral microbial community is widely regarded as a latent reservoir of antibiotic resistance genes. This study assessed the molecular epidemiology, susceptibility profile, and resistance mechanisms of 35 methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from the dental plaque of a healthy human population. Broth microdilution minimum inhibitory concentrations (MICs) revealed that all the isolates were nonsusceptible to oxacillin and penicillin G. Most of them were also resistant to trimethoprim (65.7%) and erythromycin (54.3%). The resistance to multiple antibiotics was found to be largely due to the acquisition of plasmid-borne genes. The mecA and dfrA genes were found in all the isolates, mostly dfrG (80%), aacA-aphD (20%), aadD (28.6%), aphA3 (22.9%), msrA (5.7%), and the ermC gene (14.3%). Classical mutational mechanisms found in these isolates were mainly efflux pumps such as qacA (31.4%), qacC (25.7%), tetK (17.1%), and norA (8.6%). Multilocus sequence type analysis revealed that sequence type 59 (ST59) strains comprised 71.43% of the typed isolates, and the eBURST algorithm clustered STs into the clonal complex 2-II(CC2-II). The staphyloccoccal cassette chromosome mec (SCCmec) type results showed that 25 (71.43%) were assigned to type IV. Moreover, 88.66% of the isolates were found to harbor six or more biofilm-associated genes. The aap, atlE, embp, sdrF, and IS256 genes were detected in all 35 isolates. This research demonstrates that biofilm-positive multiple-antibiotic-resistant ST59-SCCmec IV S. epidermidis strains exist in the dental plaque of healthy people and may be a potential risk for the transmission of antibiotic resistance.
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Índice:
WPRIM
Asunto principal:
Infecciones Estafilocócicas
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Staphylococcus epidermidis
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Placa Dental
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Usos Terapéuticos
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Diagnóstico
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Staphylococcus aureus Resistente a Meticilina
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Meticilina
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Microbiología
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Antibacterianos
Tipo de estudio:
Diagnostic_studies
Límite:
Female
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Humans
Idioma:
En
Revista:
International Journal of Oral Science
Año:
2020
Tipo del documento:
Article